In vivo hypermutation and continuous evolution

Directed evolution has revolutionized biomolecular engineering by applying cycles of mutation, amplification and selection to genes interest (GOIs). However, classical directed methods that rely on manually staged evolutionary constrain the scale depth search is possible. We describe genetic systems achieve rapid fully inside living cells, enabling continuous GOIs as cells grow. These advance scale, depth, ease overall power access important new areas protein engineering. In vivo a form takes advantage cells. Molina, Rix et al. discuss best practices for designing conducting experiments drug discovery, enzyme fluorescence-activated cell sorting (FACS)-based evolution.